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Seven domiciles of the urban area were selected for the entomological survey due to the presence of ecotopes appropriate to sand flies, such as chickencoops or shelters of other domestic animals, and vegetation (Fig.
The captures were performed with automatic light traps installed in the domiciles during two years between July 2012 and June 2014.
Initially the alcohol used to store the samples was discarded and 20 μl of a lyses solution (Digsol buffer) was added to each pool together with 20 μl of Proteinase K (10 mg/ml) and the samples were incubated for 3 h at 55–60 °C.
Thereafter 400 μl of ammonium acetate (4 M) was added, the samples homogenized by vortex for 15 min and centrifuged for 15 min at 13,000 rpm at 10 °C.
Further, the natural infection estimates in vectors could be used as indicator of transmission intensity to the host, hence determining risk areas.
From 1999 to 2013, there were recorded in Brazil 53,067 new cases of visceral leishmaniasis (VL), with an annual mean of 3500 new cases.
, each secreting distinct pheromones, (S)-9-methylgermacrene-B and Cembrene 1; both have been associated with different patterns of VL transmission.
The aim of the present study was to investigate the temporal distribution and natural infection of the (S)-9-methylgermacrene-B population of the The study was carried out in Panorama municipality, Sao Paulo State.
Among the insects captured a sample of live sand fly females was dissected to investigate natural infection by flagellates in their guts.
The other insects were killed by freezing for 10 min and then the samples were screened.
From 1999 to 2013 São Paulo state, recorded 2328 new cases of the disease distributed in 80 municipalities, with 202 deaths .